CHO cell lines generated by PiggyBac transposition
نویسندگان
چکیده
منابع مشابه
CHO cell lines generated by PiggyBac transposition
A major bottleneck in the manufacture of recombinant therapeutic proteins is the time and effort needed for the generation of stable, high-producing mammalian cell lines. Conventional gene transfer methods for stable cell line generation rely on random transgene integration, resulting in unpredictable and highly variable levels of expression of the transgene in individual clones [1,2]. As a con...
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Establishing stable Chinese Hamster Ovary (CHO) cells producing monoclonal antibodies (mAbs) usually pass through the random integration of vectors to the cell genome, which is sensitive to gene silencing. One approach to overcome this issue is to target a highly transcribed region in the genome. Transposons are useful devices to target active parts of genomes, and PiggyBac (PB) transposon can ...
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Human cells migrate between mother and fetus during pregnancy and persist in the respective host for long-term after birth. Fetal microchimerism occurs also in twins sharing a common placenta or chorion. Whether microchimerism occurs in multiparous mammals such as the domestic pig, where fetuses have separate placentas and chorions, is not well understood. Here, we assessed cell chimerism in li...
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Immunoglobulin A (IgA) mediates a key role in mucosal immunity and is a promising novel immunotherapeutic candidate. However, difficulties in obtaining enough material often hamper in vivo explorations. We have previously generated recombinant Chinese hamster ovary (CHO) cell lines which expressed two different HIV-1 antibodies, 3D6 and 4B3, as IgA1 [1]. One cell line (3D6-IgA) shows high produ...
متن کاملDiameters of groups generated by transposition trees
Let G = 〈S〉 be a group, and let Γ be its Cayley graph. Computing the diameter of Γ is a computationally hard problem which comes up in several contexts. Thus, it is useful to be able to compute bounds on the diameter of Cayley graphs. In [1] Ganesan studied the case where S is a minimal set of transpositions which generate G, and provided an algorithm to find an upper bound on diam Γ without ex...
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ژورنال
عنوان ژورنال: BMC Proceedings
سال: 2011
ISSN: 1753-6561
DOI: 10.1186/1753-6561-5-s8-p31